Mobile view under development, please use a desktop meanwhile

Nanobody generation & identification based on liposomes, nanodiscs or VLPs

Nanobody Core
Includes:
  • Project design and "animal welfare committee" approval
  • Immunization of a llama or an alpaca with a liposome, nanodisc or VLP
  • Peripheral Blood Lymphocytes (PBLs) preparation from immune blood
  • Total RNA preparation from the above described PBLs & QC
  • cDNA synthesis from total RNA
  • Generation of a VHH library from the cDNA & QC
  • Panning on a liposome, a nanodisc or a VLP
  • ELISA screening a liposome, a nanodisc or a VLP and a negative control
  • sequencing ELISA positive hits, B-cell lineage tagging
  • Data analysis and reporting
  • Animal care for 4 months
  • IP & commercial rights:
Deliverables:
  • Bacterial colonies harboring recombinant phagemid vector containing the Nanobody genes of the unique ELISA-positive hits
  • Report including ELISA data, Nanobodies sequence data, B-cell lineage data