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Production in inclusion bodies is a well-established strategy in Escherichia coli. Inclusion bodies are usually more resistant to proteases and thus accumulate to high amounts. Also, after isolation of the inclusion bodies, the protein of interest is already reasonable pure. However, the process of refolding the denatured proteins can be a difficult task, with low yield and laborious methods. The Core offers a 2-3 week study using a matrix screening to find the optimal refolding buffer. This matrix screen is designed in-house. Also some methods of refolding can be compared (dilution, dialysis, on-column). The deliverable is a study and an outline for a refolding process, which can also be validated.