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Despite the use of purification tags, "classical" chromatography is still needed in many steps. If no defined purification method is available, FastClean is a standardized 2-3 week study that can be offered by the VIB Protein Core in order to investigate the behavior of samples on a variety of chromatographic media, at different loading conditions. The aim is to find optimal conditions to separate the protein of interest from its contaminants. These results are used to write a purification process.