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Conjugation

Protein Core

Fluorescent molecules (antibodies) for microscopy are very expensive for research groups. When the hybridoma cell line is available for the production of an antibody, the Core can produce easily mg amounts (1-100 mg) for labeling. A lot of labeling is done with AlexaFluor groups which are easy to use. We have also expression vectors available for fusion of proteins to fluorescent proteins (GFP, mCherry). Beside fluorescent labeling; small, very stable molecules like nanobodies®, alphabodies®, affibodies® are becoming more and more important for therapeutic use. A disadvantage of these molecules is their low serum half-life. This can be increased by covalent labeling with all kinds of molecules (like PEG). The Protein Core also has expertise in this field.