The VIB Screening Core provides support in the development of miniaturized test systems/assays, which are relevant for a biological process-of-interest. In interaction with the scientists, the team of the VIB Screening Core enhances the technical reproducibility and robustness of an assay and makes it compatible with liquid handling and automation. Subsequently, a screening experiment is executed to identify substances that interfere with the assay. The aim is to accelerate the discovery of biologically active substances that can be developed into chemical probes to study biological processes and/or starting points to develop novel products for medical or agricultural applications.
The VIB Screening Core embeds the Centre for Bioassay Development and Screening (C-BIOS), a Ghent University Expertise Centre co-financed via the Special Research Fund (Bijzonder Onderzoeksfonds, BOF). C-BIOS unites the expertise and research infrastructure of VIB, Ghent University and the Cancer Research Institute Ghent (CRIG) and provides a centralized platform to efficiently support assay development and screening for the broad Life Sciences research community.
The VIB Screening Core provides services at the different levels of a screening project:
The compound collection of the VIB Screening Core amounts to a total of almost 50,000 compounds, acquired from Enamine and MicroSource Discovery Systems. The core also manages a diverse subset of the collection of CBI (Chemistry to foster Biology Innovation), a chemistry cluster that brings together chemical expertise present in the universities of Antwerp, Leuven, Ghent, Hasselt and Brussels. The total collection comprises several subcollections, from which researchers can select to screen in their project:
The VIB Screening Core has acquired the Human siGENOME/ON-TARGETplus SMARTpool siRNA library (Dharmacon, Thermo Fisher Scientific) for siRNA screening. The library comprises nine distinct subsets, and each subset is available for screening separately. The different subsets include:
a set that contains additional genes of potential therapeutic interest, including genes involved in apoptosis, senescence, nucleic acid binding, autophagy, DNA repair and characterized nuclear receptors.
Integrated screening and detection platform
The platform is a joint investment between VIB, CRIG and Ghent University. To maximize capacity and to enable assay and screening automation, the following devices are integrated in the platform:
Hu Z, Vanderhaeghen R, Cools T, Wang Y, De Clercq I, Leroux O, Nguyen L, Belt K, Millar AH, Audenaert D, Hilson P, Small ID, Mouille G, Vernhettes S, Van Breusegem F, Whelan J, Höfte H, De Veylder L. Mitochondrial Defects Confer Tolerance against Cellulose Deficiency. Plant Cell. 2016 Aug 19;28(9):2276-90. [pmid]
Xuan W*, Audenaert D*, Parizot B, Möller BK, Njo MF, De Rybel B, De Rop G, Van Isterdael G, Mähönen AP, Vanneste S, Beeckman T. Root Cap-Derived Auxin Pre-patterns the Longitudinal Axis of the Arabidopsis Root. Curr Biol. 2015 May 18;25(10):1381-8. doi: 10.1016/j.cub.2015.03.046. Epub 2015 May 7. * equal contribution [pmid]
Van de Wouwer D, Vanholme R, Decou R, Goeminne G, Audenaert D, Nguyen L, Höfer R, Pesquet E, Vanholme B, Boerjan W. Chemical Genetics Uncovers Novel Inhibitors of Lignification, Including p-Iodobenzoic Acid Targeting CINNAMATE-4-HYDROXYLASE. Plant Physiol. 2016 Sep;172(1):198-220. [pmid]
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Xuan W, Band LR, Kumpf RP, Van Damme D, Parizot B, De Rop G, Opdenacker D, Möller BK, Skorzinski N, Njo MF, De Rybel B, Audenaert D, Nowack MK, Vanneste S, Beeckman T. Cyclic programmed cell death stimulates hormone signaling and root development in Arabidopsis. Science. 2016 Jan 22;351(6271):384-7. [pmid]
Kerchev P, Mühlenbock P, Denecker J, Morreel K, Hoeberichts FA, Van Der Kelen K, Vandorpe M, Nguyen L, Audenaert D, Van Breusegem F. Activation of auxin signalling counteracts photorespiratory H2O2-dependent cell death. Plant Cell Environ. 2015 Feb;38(2):253-65. [pmid]