Metabolomics provides a functional readout of the cells biochemistry. It holds great potential to link the observed differences in the phenotype to the underlying biochemical and molecular mechanisms.
The Metabolomics Core at VIB consists of two platform sites, located in Ghent and Leuven. Both sites provide contemporary mass spectrometry (MS) based metabolomic services and offer customized solutions for your experimental setup if requested.
The site in Ghent is embedded in the VIB-UGent Center for Plant Systems Biology and specializes in secondary metabolism. By constructing a unique in house library with over 5000 secondary metabolites, the team developed a high throughput pipeline for comparative metabolic profiling. The introduction of Ion Mobility Mass Spectrometry and the use of advanced data processing software (including basic statistics) ensures a straightforward and high quality data output. Additional characterization through compound purification and NMR analysis are also available, but optional.
The site in Leuven is linked to the VIB-KU Leuven Center for Cancer Biology and specializes in primary metabolism. It also holds unique expertise in tracer (13C, 15N, 18O, 2H, etc) based metabolomics, this technology provides crucial insights into the activity of metabolic pathways by administration of non-radioactive isotopically labeled substrates (such as glucose, glutamine, palmitic acid, etc) to the medium (in vitro) or organism (in vivo). Using state-of-the-art high resolution mass spectrometry, the fate of these labeled isotopes is followed throughout the metabolic network and it reveals the (in)activity of metabolic pathways and enzymes. The team is specialized in the experimental design, data analyses and visualization of tracer metabolomics. The team also provides services for metabolic profiling of primary metabolites (using an in house library of over 400 metabolites).
All services are open to the VIB community, academic researchers and industry. We are always willing to assist you in formulating biological conclusions, if needed. To always ensure a high quality service, we constantly invest in the following up and implementation of novel and cutting-edge techniques for metabolomics.
The Metabolomics Core provides mass spectrometry-driven metabolomics, and our services include, but are not limited to:
The Metabolomics Core has immediate access to the following state-of-the-art mass spectrometers: